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Heterologous Protein Production in CHO Cells: Methods and Protocols / Meleady, Paula, ed.

Tác giả : Meleady, Paula, ed.

Nhà xuất bản : Humana Press

Năm xuất bản : 2017

ISBN : 978-1-4939-6972-2

Tùng thư : Methods in molecular biology

Chủ đề : 1. Biotherapeutics. 2. Cells. 3. Molecular Biology. 4. Book.

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Tóm tắt :

This book includes reviews and protocols for genetic manipulation of CHO cells for recombinant protein production, including “difficult-to-express” therapeutics. A method is also included on the use of the recently described genome editing tool, CRISPR/Cas9, and how this can be applied to CHO cells. The book also includes a review and protocols for characterization of CHO cells using ‘omic approaches and how these methods can be used to improve efficiency of recombinant protein production during cell line development.

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http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/57300